Project Overview

The dual-electrode glutamine sensor project aims to develop an accurate, reliable electrochemical sensor for measuring glutamine in blood samples. This sensor addresses a critical challenge in glutamine detection: interference from glutamate, a structurally similar amino acid often present in biological fluids.

By employing a differential measurement approach with two working electrodes, the sensor can effectively eliminate glutamate interference and provide accurate glutamine measurements in complex biological matrices.

Key Innovations

Dual-Electrode Design

Two working electrodes on a single substrate enable differential measurement to eliminate glutamate interference.

Nanomaterial Enhancement

Carbon nanotubes and copper oxide nanoparticles improve sensitivity and electron transfer.

Enzyme Immobilization

Optimized cross-linking techniques for stable enzyme layers with high activity retention.

Selective Membranes

Multi-layer membrane system for interference rejection and anti-fouling properties.

Applications

Accurate glutamine measurement has important applications in:

About This Website

This website provides comprehensive, step-by-step protocols for scientists and researchers to prototype the dual-electrode glutamine sensor in a laboratory setting. The protocols cover all aspects of sensor development, from materials sourcing to functional testing and validation.

The content is organized into logical sections following the sensor development workflow, with detailed instructions, troubleshooting guidance, and scientific references to support successful implementation.

Note: This website and the protocols contained herein are provided for scientific research purposes only. The sensor design has not been approved for clinical diagnostic use.